2.7.1.68	-	
2.7.1.68	amylose-resin column chromatography	
2.7.1.68	cytosolic enzyme type II enzyme and membrane-bound type I and type II enzyme	
2.7.1.68	glutathione-Sepharose chromatography	
2.7.1.68	glutathione-Sepharose column chromatography	
2.7.1.68	Ni-NTA agarose column chromatography	
2.7.1.68	Ni-NTA agarose column chromatography or glutathione-Sepharose column chromatography	
2.7.1.68	partial	
2.7.1.68	Partial purification from Escherichia coli culture cells. The cells are suspended in PBS buffer, sonicated on ice, and centrifuged. Supernatant is mixed with glutathione-Sepharose 4B and incubated at 48°C for 2 h. The Sepharose beads are collected and washed with an excess volume of PBS.	
2.7.1.68	recombinan GST-fusion isozyme alpha from Escherichia coli