2.7.1.160	by Blue Sepharose column chromatography	
2.7.1.160	by nickel affinity chromatography	
2.7.1.160	His6-tagged wild-type RslTpt1 and mutant R16A/H17A/R64A/R119A RslTpt1 from Escherichia coli strain L21(DE3)-CodonPlus by nickel affinity chromatography, ultrafiltration, and gel filtration	
2.7.1.160	immobilized metal ion affinity chromatography, > 80% purified by SDS-PAGE	
2.7.1.160	KptA-His6 10% purified by sonication, immobilized metal ion chromatography, centrifugation	
2.7.1.160	Ni-agarose chromatography	
2.7.1.160	partially purified	
2.7.1.160	partially purified as a His6-tagged protein	
2.7.1.160	partially purified by Blue sepharose column chromatography	
2.7.1.160	partially purified by chromatography on heparin-agarose