2.5.1.86 - 704266 2.5.1.86 Ni-NTA column chromatography and HiPrep 16/60 S-100 gel filtration 758604 2.5.1.86 recombinant GST-tagged subunit from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, tag cleavage with factor Xa, and ultrafiltration 737596