2.4.99.19 recombinant His-tagged full-length PglB from Escherichia coli strain BL21(DE3) in the membrane fraction by ultracentrifugation, recombinant GST-tagged wild-type and selenomethionine-labeled C-terminal globular domain of PglB from Escherichia coli strain BL21(DE3)pLysS by glutathione affinity chromatography and cleavage of the tag by 3C protease, followed by to reductive methylation of the lysine residues, gel filtration, and anion exchange chromatography 709145 2.4.99.19 recombinant His-tagged PglB from Escherichia coli strain C43(DE3) by nickel affinity chromatography 707399 2.4.99.19 recombinant His10-tagged wild-type and mutant enzymes from Escherichia coli strain SCM6 720542