2.4.1.83	-	
2.4.1.83	DEAE-cellulose column chromatography, gel filtration	
2.4.1.83	His-tag fusion protein purified using ProBond purification system, more than 97% pure	
2.4.1.83	one-step purification	
2.4.1.83	partial	
2.4.1.83	recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain C41(DE3) membranes by solubilization with 1% n-dodecyl-beta-D-maltoside in presence of 5% v/v glycerol, nickel affinity chromatography, followed by gel filtration and ultrafiltration. Mutant variant DELTA230-352 is prepared as two fractions, one purified from the membrane fraction (DELTA230-352 m) and one from the aqueous phase (DELTA230-352 s)	
2.4.1.83	solubilized with Triton X-100 and purified to homogeneity