2.3.1.31	-	486779, 672001, 673497	
2.3.1.31	cells are centrifuged in 50 mM Tris, pH 8.0, sonicated, centrifuged, supernatant loaded to a Ni2+-nitrilotriacetic acid-agarose column, washed, and eluted, eland incubated with protease and dialyzed, reloaded onto a Ni2+-nitrilotriacetic acid-agarose column, dialyzed, loaded onto Superdex-200 size-exclusion fast protein liquid chromatography column with 25 mM HEPES-buffer, pH 7.5	687796	
2.3.1.31	partial	486780, 486781, 486782, 486786	
2.3.1.31	partial, mutant strain 6	486784	
2.3.1.31	recombinant from E. coli	486788	
2.3.1.31	recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion excange chromatography, followed by gel electrophoresis, dialysis, and ultrafiltration	735393	
2.3.1.31	recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)-T1 by nickel affinity chromatography and two different steps of gel filtration	755982	
2.3.1.31	recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography, dialysis and ultrafiltration	736358	
2.3.1.31	using Ni-NTA chromatography	684480	
2.3.1.31	using Ni-NTA-chromatography	687796