2.3.1.1 - 2.3.1.1 ammonium sulfate, DEAE Biogel, Sephacryl S-200, aminoacetyl biogel A, affi-gel blue, sucrose gradient, isoelectric focusing 2.3.1.1 ammonium sulfate, hydroxyapatite 2.3.1.1 ammonium sulfate, hydroxyapatite, 10fold purification 2.3.1.1 ammonium sulfate, hydroxyapatite, DEAE-cellulose, Sephacryl-300 2.3.1.1 ammonium sulfate, hydroxyapatite, DEAE-cellulose, Sephadex G-100 2.3.1.1 DEAE-cellulose, hydroxylapatite 2.3.1.1 gel filtration 2.3.1.1 HisTrap nickel affinity column chromatography 2.3.1.1 HiTrap column chromatography