2.1.1.74	-	
2.1.1.74	purified to homogeneity by metal affinity or ion exchange and heparin affinity, respectively, followed by gel filtration	
2.1.1.74	recombinant enzyme from Escherichia coli strain BL21(DE3) by anion and cation exchange chromatography and gel filtration to over 95% purity	
2.1.1.74	recombinant enzyme from Escherichia coli to homogeneity	
2.1.1.74	using Ni-NTA chromatography