2.1.1.265 recombinant His-tagged enzyme with a TEV protease cleavage site from Escherichia coli strain BL21(DE3) PlysS by nickel affinity chromatography, cleavage of the His-tag, followed by gel filtration 718496 2.1.1.265 recombinant His6-tagged wild-type TehB from Escherichia coli strains C41(DE3) and BL21(DE3), respectively, by nickel affinity chromatographhy 719697