1.8.2.4 ammonium sulfate precipitation, DEAE-Sepharose column chromatography, hydroxyapatite column chromatography, and Sephacryl S-200 gel filtration 714886 1.8.2.4 ammonium sulfate precipitation, SP-Sepharose column chromatography, gel filtration 715664 1.8.2.4 Poros 20HQ Strong anion exchange column chromatography and gel filtration 716248