1.8.1.B1	-	
1.8.1.B1	2',5'-ADP Sepharose column chromatography and Sephadex G-25 gel filtration	
1.8.1.B1	ADP-agarose column chromatography	
1.8.1.B1	ammonium sulfate precipitation, Ni-NTA column chromatography, GSH-Sepharose column chromatography, and 2',5'-ADP-Sepharose column chromatography	
1.8.1.B1	DEAE-Sephacel gel filtration, hydroxyapatite chromatography column chromatography, and Cibacron blue chromatography column chromatography	
1.8.1.B1	Ni-NTA column chromatography	
1.8.1.B1	nickel affinity column chromatography	
1.8.1.B1	recombinant enzyme	
1.8.1.B1	recombinantly produced human enzyme (hTGR), the Sec->Cys mutant (hTGRU642C) of the full-length protein, the isolated TrxR domain (hTGR151-643) and the Grx domain containing a monothiol active site (hTGR1-150) are produced	
1.8.1.B1	small amounts of the soluble enzyme are obtained by purifying it from a large quantity of cells, in which protein synthesis is induced slowly for a period of 24 h at a low temperature