1.6.5.4	-	
1.6.5.4	Butyl-Toyopearl column chromatography, HiTrap Q column chromatography and Blue Sepharose 6FF column chromatography	
1.6.5.4	glutathione Sepharose column chromatography	
1.6.5.4	Ni-NTA agarose chromatography	
1.6.5.4	Phenyl Superose gel filtration, Blue Sepharose column chromatography and DEAE cellulose column chromatography	
1.6.5.4	recombinant enzyme	
1.6.5.4	recombinant from Escherichia coli	
1.6.5.4	several native enzyme forms from lens soluble fraction by gel filtration, affinity chromatography, native isoelectric focusing, and 2D-electrophoresis, about 1400fold