1.4.1.20 - 349633, 349634, 349636, 349637, 349638, 349640, 349650, 349652 1.4.1.20 best purification results of recombinant PheDH are achieved in an polymer/salt aqueous two-phase systems (ATPS) with 9% of phenazine ethosulfate with a molecular mass of 6000 Da (PEG-6000) and 16% of K2HPO4 696787 1.4.1.20 construction of a chimeric enzyme consisting of the N-terminal domain of Thermoactinomyces intermedius phenylalanine dehydrogenase, containing the substrate-binding region and the C-terminal domain of leucine dehydrogenase from Bacillus stearothermophilus containing the NAD+-binding region 349647 1.4.1.20 in aqueous two-phase systems composed of polyethylene glycol 6000 and ammonium sulfate with 8.5% (w/w) PEG-6000, 17.5% (w/w) (NH4)2SO4 and 13% (w/w) NaCl at pH 8.0 687951 1.4.1.20 mutant enzyme F124M/V125S/H126I/A127I/A128Y/R129Q 349649 1.4.1.20 Ni-affinity and anion exchange column chromatography 686443 1.4.1.20 Ni-affinity column chromatography 685742 1.4.1.20 Ni-affinity column chromatography and anion exchange column chromatography 690070 1.4.1.20 Ni-IDA column chromatography 743108 1.4.1.20 partial 349635