1.23.5.1 - 660062, 660205, 660276 1.23.5.1 in presence of Tween 20. The enzyme has more than one disulfide bond and takes multiple forms depending on the extent of the reduction 660138 1.23.5.1 native enzyme partially by preparation of thylakoid membranes, further purifications of the VDE by, i.e. anion exchange chromatography and gel filtration, lead to a significant loss of VDE activity 726106 1.23.5.1 nickel affinity column chromatography 712452 1.23.5.1 recombinant enzyme from Escherichia coli strain BL21(DE3) solubilized from inclusion bodies 745996 1.23.5.1 recombinant tagged wild-type and mutant enzymes from Escherichia coli strain Origami B by affinity chromatography 744904 1.23.5.1 recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) solubilized from inclusion bodies 745983 1.23.5.1 TALON metal affinity resin column chromatography 728500