1.2.1.67 by DEAE-Sepharose FF column, butyl-Sepharose column and Resource Q column chromatography 725035 1.2.1.67 DEAE-Sepharose column chromatography, Resource Phe column chromatography, and Mono Q column chromatography 762865 1.2.1.67 partially purified by (NH4)2SO4 precipitation but it losts activity during attempted chromatographic steps 724018 1.2.1.67 purified to apparent electrophoretic homogeneity using His SpinTrap columns 724040 1.2.1.67 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration 743835