1.2.1.67	by DEAE-Sepharose FF column, butyl-Sepharose column and Resource Q column chromatography	
1.2.1.67	DEAE-Sepharose column chromatography, Resource Phe column chromatography, and Mono Q column chromatography	
1.2.1.67	partially purified by (NH4)2SO4 precipitation but it losts activity during attempted chromatographic steps	
1.2.1.67	purified to apparent electrophoretic homogeneity using His SpinTrap columns	
1.2.1.67	recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration