1.2.1.47 14.2fold to apparent homogeneity, by hydrophobic chromatography and affinity chromatography 691376 1.2.1.47 ammonium sulfate precipitation, phenyl-Toyopearl column chromatography, and 5'-AMP Sepharose column chromatography 726537 1.2.1.47 recombinant FLAG-tagged enzyme TMABA-DH from HEK-293T cells by immunoaffinity chromatography 743576 1.2.1.47 recombinant His-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) pLysS by cobalt affinity and nickel affinity chromatography, followed by ultrafiltration and gel filtration 762831 1.2.1.47 recombinant SUMO-His6-tagged ALDH9A1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the SUMO-His tag, dialysis, ultrafiltration, and gel filtration 762675 1.2.1.47 using column chromatography on blue dextran-Sepharose and 5'-AMP-Sepharose 288254 1.2.1.47 using column chromatography on SP-Sepharose, Red Sepharose CL-6B and Hydroxylapatite 288255