1.14.99.39	-	
1.14.99.39	native enzyme 9.7fold by by anion-exchange chromatography and gel filtration as membrane-bound monomer	
1.14.99.39	native enzyme by anion exchange chromatography and gel filtration	
1.14.99.39	recombinant C-terminally Strep-tagged protein, comprising amino acids 31-186, from Escherichia coli strain Rosetta-2 (DE3) by affinity chromatography, ultrafiltration, and gel filtration	
1.14.99.39	the free ethynyl group of the inactive enzyme-inactivator adduct is conjugated with either a visualization tag (e.g., Alexa Fluor 647 azide) or an affinity purification tag (e.g., biotin-azide) using a copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction. The resulting enzyme-probe-tag conjugant can then either be (i) visualized using IR fluorescence in SDS-PAGE or (ii) enriched by affinity chromatography, tryptically digested, and identified by LC-MS/MS