1.14.15.16 - 1.14.15.16 recombinant active His-tagged enzyme from Escherichia coli membranes by nickel affinity and hydrophobic interaction chromatography and dialysis, adrenodoxin-affinity chromatography is not successful 1.14.15.16 recombinant N-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli strain DH5alpha F'IQ by nickel affinity chromatography and gel filtration, copurification with added recombinant bovine adrenodoxin for enzyme-cofctor complex preparation 1.14.15.16 recombinant N-terminally maltose-binding-protein fused enzyme from Escherichia coli by affinity chromatography to homogeneity 1.14.15.16 Talon metal affinity column chromatography