1.14.13.128 native heteroxanthine demethylase partially 1.14.13.128 Ni-NTA column chromatography 1.14.13.128 Ni-NTA column chromatography and Sephacryl S200 gel filtration 1.14.13.128 partial 1.14.13.128 purification of two-subunit N-demethylase component (Ndm) of the N-demethylase holoenzyme 1.14.13.128 recombinant His-tagged wild-type and mutant NdmC proteins by nickel affinity chromatography and gel filtration