1.13.11.71 a small amount of recombinant, catalytically inactive His-tagged enzyme from Escherichia coli strain BL21-Gold (DE3) by cobalt affinity chromatography 1.13.11.71 native enzyme 9.5fold to homogeneity by ammonium sulfate fractionation and gel filtration 1.13.11.71 recombinant His-tagged enzyme from Escherichia coli strain BL21-Gold (DE3) by cobalt affinity chromatography