1.11.2.3	DEAE-Trisacryl 300 gel filtration and CM-Sepharose column chromatography	
1.11.2.3	DEAF-cellulose column chromatography and agarose gel column chromatography	
1.11.2.3	Ni-NTA column chromatography	
1.11.2.3	Ni2+-affinity column chromatography	
1.11.2.3	nickel agarose bead chromatography and HiTrap Q FF Sepharose column chromatography