1.1.1.45 - 286905, 286907, 695345 1.1.1.45 12%-52% yield 286911 1.1.1.45 67% yield 286906 1.1.1.45 ammonium sulfate fractionation, Sephadex G-100 column chromatography, Q-Sepharose column chromatography, Blue-Sepharose column chromatography, hydroxylapatite column chromatography 669147 1.1.1.45 recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) cells 712765