1.1.1.202 - 1.1.1.202 by centrifugation, nickel affinity column and gel filtration 1.1.1.202 Ni-NTA column chromatography 1.1.1.202 recombinant His-tagged enzyme 7.1-7.3fold from Escherichia coli strain JM109 by nickel affinity chromatography and gel filtration 1.1.1.202 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration 1.1.1.202 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration 1.1.1.202 recombinant protein 1.1.1.202 recombinant soluble enzyme from Escherichia coli strain BL21(DE3) 1.1.1.202 recombinant tagged selenomethionine-labeled enzyme from Escherichia coli by nickel affinity, ion exchange, and gel filtration chromatography and ultrafiltration 1.1.1.202 soluble recombinant His-tagged enzyme 6.6-6.7fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration to homogeneity