1.1.1.156 DEAE-cellulose column chromatography, hydroxyapatite column chromatography, BD Sepharose column chromatography, and DEAE-Sephadex A-50 gel filtration 1.1.1.156 partial 1.1.1.156 to homogeneity, 1step chromatography 1.1.1.156 to homogeneity, chromatography techniques