1.1.1.122 DEAE-Sepharose column chromatography, phenyl-Toyopearl column chromatography, Q-Sepharose column chromatography, Superdex 200HR gel filtration, and Mono Q column chromatography 1.1.1.122 Ni2+ affinity chromatography 1.1.1.122 to homogeneity, chromatography steps, preparative gel electrophoresis 1.1.1.122 to homogeneity, chromatography steps, preparative gel electrophoresis, affinity chromatography 1.1.1.122 to homogeneity, chromatography techniques