5.1.99.1	-	2434, 2437, 2438, 2439, 2441, 2442, 649408, 726729	
5.1.99.1	lysate is clarified by centrifugation and purified by passage through a nickel-affinity column, and a further purification step is performed by gel filtration	706660	
5.1.99.1	recombinant enzyme	693174	
5.1.99.1	recombinant MCE-1	661687	
5.1.99.1	recombinant N-terminally His-tagged enzyme from Escherichia coli strain Rosetta 2 (DE3) by nickel affinity chromatography	726729