4.1.99.5 - 713511, 715293, 715300 4.1.99.5 208fold, solubilized with octyl beta-glucoside 648394 4.1.99.5 partial, 1306fold, solubilized with beta-octyl glucoside 648395 4.1.99.5 recombinant enzyme from Escherichia coli 726552, 747074 4.1.99.5 recombinant enzyme mutants V41Y and A134F to homogeneity 727312 4.1.99.5 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) in an anaerobic chamber, further by metal affinity chromatography 727690 4.1.99.5 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration, followed by ultrafiltration 749212 4.1.99.5 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography and dialysis 749051 4.1.99.5 recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by anion exchange chromatography and dialysis 726548 4.1.99.5 recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, removal of the tag by thrombin, and gel filtration 727215