4.1.2.20 - 5078, 5083, 649100, 716878 4.1.2.20 ammonium sulfate precipitation, phenyl-Sepharose column chromatography, UnoQ5 column chromatography, and Superdex 200 gel filtration 704334 4.1.2.20 DEAE-Sepharose column chromatography and Superdex 75 gel filtration 677338 4.1.2.20 homogeneity 650974 4.1.2.20 using metal affinity and size exclusion chromatography 691007