4.1.1.112 - 4263, 4268, 4269, 4272, 4273, 4276, 4283, 653877, 654328, 663795, 692317, 715662, 748206, 756006 4.1.1.112 ammonium sulfate precipitation, HiPrep column chromatography, Mono Q column chromatography, and Superdex-200 gel filtration 704327 4.1.1.112 avidin-Sepharose column chromatography 716673 4.1.1.112 DEAE-cellulose column chromatography, phenyl-Sepharose columnn chromatography, and butyl-Sepharose column chromatography 678331 4.1.1.112 enzyme from Vibrio cholerae and heterologously expressed enzyme 663796 4.1.1.112 Ni-NTA column chromatography and Superdex G-200 gel filtration 713674 4.1.1.112 Ni2+-bounded affinity column chromatography 714979 4.1.1.112 Ni2+-NTA-agarose column chromatography 681424 4.1.1.112 partial 4270, 4271 4.1.1.112 recombinant 664936