3.5.5.5	-	32503, 667345, 755864	
3.5.5.5	ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Sephacryl S-300 gel filtration	733546	
3.5.5.5	cells of bacteria culture are disrupted and centrifugation and the enzyme in the soluble fraction is purified using Ni-NTA affinity chromatography	694107	
3.5.5.5	DEAE Sepharose Fast Flow column chromatography and Sephacryl S-300 gel filtration	687882	
3.5.5.5	Ni-NTA column chromatography	733558	
3.5.5.5	partial, 259fold	652959	
3.5.5.5	recombinant enzyme 2.4fold from Escherichia coli strain BL21-Gold(DE3) to near homogeneity	718670	
3.5.5.5	recombinant enzyme 3fold from Escherichia coli strain BL21-Gold(DE3) to near homogeneity	718670	
3.5.5.5	recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	719629