3.4.24.84 IgG Sepharose 6 column chromatography, nickel affinity resin column chromatography, and Superdex S200 gel filtration 755334 3.4.24.84 Ni-bead-purified 667497 3.4.24.84 recombinant His-tagged Ste24, nickel chelate chromatography 638977