3.2.1.145	ammonium sulfate precipitation, DEAE-Toyopearl 650-M column chromatography and Resource PHE column chromatography	
3.2.1.145	DEAE-cellulose, CM-Toyopearl, hydroylapatite, electrofocusing, purified from commercial driselase	
3.2.1.145	native enzyme 1.8fold from culture supernatant by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration	
3.2.1.145	Ni-nitrilotriacetic acid agarose colum	
3.2.1.145	Ni-NTA agarose column	
3.2.1.145	recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3)	
3.2.1.145	recombinant enzyme 0.7fold from Pichia pastoris cell culture medium by cation exchange chromatography and gel filtration	
3.2.1.145	recombinant enzyme expressed in Pichia pastoris	
3.2.1.145	recombinant expression of His-tagged wild-type and selenomethionine-labeled enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration	
3.2.1.145	recombinant selenmethionine-labeled Pc1,3Gal43A from Pichia pastoris by ultrafiltration, and hydrophobic interaction chromatography