2.7.1.175 native enzyme by heat treatment at 50°C for 30 min, anion exchange chromatography, ammonium sulfate fractionation, hydrophobic interaction chromatography, ultra- and gel filtration, and another step of anion exchange chromatography 717163 2.7.1.175 recombinant C-terminally His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, followed by gel filtration 739666 2.7.1.175 recombinant enzyme from Escherichia coli by anion exchange chromatography and gel filtration 717432 2.7.1.175 recombinant His-tagged enzyme from Mycobacterium smegmatis by nickel affinity chromatography 737320 2.7.1.175 recombinant His-tagged enzyme from Streptomyces lividans strain 66 by nickel affinity chromatography 717739