2.6.1.18 heat treatment, alumina C-gamma gel, protamine sulfate, ammonium sulfate 288059 2.6.1.18 K-22, partial purification 636596 2.6.1.18 nickel column chromatography 672713 2.6.1.18 nickel Sepharose Hi-Performance column chromatography and Superdex 200 Hiload 16/60 gel filtration 671123 2.6.1.18 recombinant His-tagged enzyme from Escherichia coli 759951 2.6.1.18 recombinant His-tagged enzyme from Escherichia coli strain BL21 657597 2.6.1.18 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 759802 2.6.1.18 recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration 759057 2.6.1.18 using an Ni-NTA affinity column 723317 2.6.1.18 using Ni-NTA chromatography 721893