2.6.1.15 ammonium sulfate fractionation , DE-52 chromatography 639898 2.6.1.15 DE-52 chromatography, hydroxyapatite chromatography 639901 2.6.1.15 DE-52 chromatography, hydroxyapatite chromatography for soluble fraction, DE-52 chromatography for mitochondrial fraction 639897 2.6.1.15 recombinant His-tagged enzyme from Escherichia coli by nickel affinity purification, two different steps of anion exchange chromatography, and gel filtration 746952