2.1.1.274 by DE53 DEAE anion exchange, HAP and Mono-Q anion exchange chromatography 721412 2.1.1.274 by nickel-based affinity chromatography 721415 2.1.1.274 N-terminal polyhistidine-tagged SAMT protein purified by Ni2+ affinity chromatography and gel filtration chromatography using a Superdex-75 column 723402 2.1.1.274 recombinant enzyme CsSAMT from Escherichia coli and from Saccharomyces cerevisiae 757035 2.1.1.274 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) Codon Plus by metal affinity chromatography 736842 2.1.1.274 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 757984 2.1.1.274 to near homogeneity in two steps, first using anion-exchange column diethylaminoethyl cellulose followed by Mono-Q anion-exchange chromatography 723416