2.1.1.170 - 2.1.1.170 recombinant FLAG-tagged RsmG from Escherichia coli TOP10 cells by affinity chromatography 2.1.1.170 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 2.1.1.170 the AdoMet cofactor is tightly bound in RsmG and copurifies with the recombinant protein