1.8.4.13 native enzyme about 1100fold by ammonium sulfate fractionation, anion exchange chromatography, gel filtration, again anion exchange chromatography, heat treatment, and hydroxyapatite chromatography to near homogeneity 393068 1.8.4.13 native enzyme partially by subcellular fractionation 393071 1.8.4.13 two native enzyme forms by ammonium sulfate fractionation, anion exchange chromatography, and a calcium phosphate resin chromatography 393070