1.4.4.2 - 391978, 391979, 391980, 391984, 391990, 391993, 391994, 391996, 654089, 686720 1.4.4.2 H1, H2, and L protein purified to homogeneity, more than 95% pure 673411 1.4.4.2 Ni-NTA column chromatography 724197 1.4.4.2 Q-Sepharose column chromatography and Sephacryl S-100 gel filtration 697916 1.4.4.2 recombinant His-tagged enzyme from Escherichia coli by nickel affinity and ion exchange chromatography, and gel filtration 710748 1.4.4.2 recombinant His6-tagged enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis 741906 1.4.4.2 ultracentrifugation 688637 1.4.4.2 using a nickel-nitrilotriacetic acid-agarose column 673411