1.3.98.1 centrifugation after cell disruption, separation from the NADP-linked dihydroorotic dehydrogenase 390570 1.3.98.1 preparative gel electrophoresis 390909, 390911 1.3.98.1 acid treatment, DE-52-, Matrex Orange A-, Matrex Blue A column chromatography 390910 1.3.98.1 streptomycin sulfate and ammonium sulfate precipitation, DEAE-Sephacel-, affinity chromatography 390910 1.3.98.1 ion-exchange, gel filtration, ammonium sulfate precipitation 390911 1.3.98.1 streptomycin precipitation, ion-exchange, hydroxylapatite 390916 1.3.98.1 Orange-A column, reverse-affinity step, Mono Q column 390927 1.3.98.1 - 390916, 390927, 391222, 656747, 684914, 685158 1.3.98.1 partial 655517 1.3.98.1 native enzyme from the cytosolic fraction by gel filtration and immunoaffinity chromatography, recombinant His-tagged enzyme by nickel affinity chromatography to homogeneity and subsequent clevage of the tag by thrombin 656747