1.14.99.53	-	
1.14.99.53	expression Bacillus subtilis yields a simple purification process without complicated periplasmic fractionation, as well as improved productivity	
1.14.99.53	improved expression and purification protocol using Champion pET-SUMO vector, yielding high purity AA10 with yields in excess of 9 mg of protein per litre of culture	
1.14.99.53	protocol for cloning, expression and purification of lytic cellulose monooxygenase (EC 1.14.99.54) and lytic chitin monooxygenase (EC 1.14.99.53), especially from termites, silkworm and honeybee	
1.14.99.53	recombinant His-tagged protein	
1.14.99.53	recombinant protein	
1.14.99.53	simple purification method by affinity adsorption to obtain functional lytic polysaccharide monooxygenases. Purification follows a binding-elution protocol with low-grade polysaccharides including Avicel