1.14.15.8	-	
1.14.15.8	it is possible to resolve the hydroxylase system into three proteins: a strictly NADPH-dependent FMN-containing flavoprotein (megaredoxin reductase), an iron-sulfur protein (megaredoxin), and cytochrome P-450 (P-450meg). The activity of the 15beta-hydroxylase system is fully reconstituted upon combination of these three proteins and addition of NADPH. Megaredoxin has an apparent sulfur to iron ratio of 0.98 and shows g-signals at 1.90, 1.93, and 2.06 when analyzed by electron paramagnetic resonance spectroscopy	
1.14.15.8	the purification procedure includes chromatography on DEAE-cellulose, Ultrogel ACA-54, DEAE-Sepharose, octyl-Sepharose, and hydroxyapatite