1.14.11.6 - 439135, 439137, 439140, 439144, 439146 1.14.11.6 native enzyme by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration. Recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, the tag is cleaved off by thrombin, followed by gel filtration 697315 1.14.11.6 partial 439138 1.14.11.6 recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) Condon Plus by nickel affinity chromatography and gel filtration 745921