1.13.11.77	Q Sepharose column chromatography and Macro-Prep SE 100/40 gel filtration	
1.13.11.77	recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography	
1.13.11.77	recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis