1.13.11.60 - 1.13.11.60 His-Trap column chromatography 1.13.11.60 partial 1.13.11.60 recombinant His-tagged wild-enzyme from Escherichia coli strain ER2566 by nickel affinity chromatography 1.13.11.60 recombinant His-tagged wild-type and mutant enzymes 8fold from Escherichia coli strain ER2566 by nickel affinity chromatography