1.1.99.22 - 287791 1.1.99.22 CM-Toyopearl C-650 column chromatography, and Sephadex G-200 gel filtration 740188 1.1.99.22 n-octyl-beta-D-thioglucoside, n-octyl-beta-D-glucoside, Mydol 10, and Mydol 12 are effective detergents to solubilize the enzyme 760731 1.1.99.22 to homogeneity by anion exchange chromatography and Ni-affinity chromatography 674959 1.1.99.22 to homogeneity by Ni-affinity chromatography 668061