5.6.1.5 - 5.6.1.5 26S proteinase 5.6.1.5 26Sproteasome 5.6.1.5 700-kDa complex 5.6.1.5 full length protein, the interdomain Mpa-ID and the mutant forms 5.6.1.5 gel filtration 5.6.1.5 glutathione Sepharose column chromatography and Ni-NTA resin column chromatography 5.6.1.5 glutathione-Sepharose column chromatography 5.6.1.5 His-tag purification resin chromatography, MonoQ column chromatography, and Superose 6 gel filtration 5.6.1.5 HiTrap Q column chromatography and Superose 12 gel filtration 5.6.1.5 M2 agarose affinity column chromatography 5.6.1.5 Mpa fragments are purified as N-terminal fusions with His6-tagged maltose-binding protein followed by a TEV cleavage site, producing the folowing fragments: Mpa-wt (residues 1-609), Mpa-CC (residues 1-98), Mpa-ID (99-216), and Mpa-CC-ID (1-216) 5.6.1.5 NeutrAvidin agarose column chromatography and Superose 6 gel filtration 5.6.1.5 NeutrAvidin agarose resin column chromatography and Superose 6 gel filtration 5.6.1.5 NeutrAvidin agarose resin column chromatography, and Superose 6 gel filtration 5.6.1.5 Ni-NTA column chromatography and Superdex 200 gel filtration 5.6.1.5 proteasomes are prepared from rabbit reticulocyte lysates 5.6.1.5 proteins are extracted from heart tissue 5.6.1.5 proteins are extracted from pupae and larvae of Drosophila melanogaster 5.6.1.5 purified in the presence of ATP-gammaS and ATP 5.6.1.5 recombinant expression of His-tagged C-terminal ATPase domain of Rpt5 (residues 173-442) from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, and gel filtration as protein complex with the N-terminus of p27 5.6.1.5 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 5.6.1.5 recombinant PAN, expressed in Escherichia coli Bl21(DE3) 5.6.1.5 recombinant rat SuG1, produced in Escherichia coli 5.6.1.5 recombinant Rpt subunits 5.6.1.5 recombinant Rpt2 5.6.1.5 the Nas6-Rpt3C complex, expressed in Escherichia coli Rosetta DE3 cells, is purified on a HisTrap HP, a HiTrapQ anion-exchange, and a gel-filtration column 5.6.1.5 ultracentrifugation and MonoQ column chromatography