5.4.99.30 - 702770, 703883 5.4.99.30 purified 94fold using hydrophobic chromatography and gel filtration 729435 5.4.99.30 recombinant His-MBP-tagged isozyme RGP1 from Escherichia coli strain BL21 Gold by nickel affinity chromatography, tag cleavage with TEV protease, followed by another step of nickel affinity chromatography, and gel filtration 747199 5.4.99.30 recombinant His-MBP-tagged isozyme RGP2 from Escherichia coli strain BL21 Gold by nickel affinity chromatography, tag cleavage with TEV protease, followed by another step of nickel affinity chromatography, and gel filtration 747199 5.4.99.30 recombinant His-MBP-tagged isozyme RGP3 from Escherichia coli strain BL21 Gold by nickel affinity chromatography, tag cleavage with TEV protease, followed by another step of nickel affinity chromatography, and gel filtration 747199 5.4.99.30 recombinant His-tagged wild-type and mutant isozymes UAM1 from Escherichia coli strain BL21 by nickel affinity chromatography 747199