5.3.99.4	-	
5.3.99.4	partial	
5.3.99.4	recombinant C-terminally His4-tagged modified enzyme from Escherichia coli strain BL21/(DE3)pLys by nickel affinity chromatography and carboxymethyl cation exchange chromatography to homogeneity	
5.3.99.4	recombinant enzyme	
5.3.99.4	recombinant enzyme is purified to homogeneity	
5.3.99.4	to electrophoretic homogeneity